According to the identification of disease resistance, the resistance function of the candidate gene TaFBN was further verified. These two genes were further verified by Agrobacterium-mediated wheat genetic transformation technology. Two of the candidate genes temporarily designated as TaFBN and Ta_Pes_BRCT, produced the most significant resistance changes in the wheat- Pst interaction system after silencing.
Herein, based on previous YrM8664-3 chromosome location results (bin 4AL13-0.59-0.66 close to 4AL12-0.43-0.59) and expression change information of candidate genes and bioinformatics analysis, several candidate genes with significantly different expression changes were then selected and verified by virus-induced gene silencing (VIGS). A single dominant gene temporarily designated as YrM8664-3, from a wheat- Leymus mollis introgression line M8664-3 highly resistant to Chinese predominant Pst races, is a potentially valuable source of stripe rust resistance for breeding. Therefore, it is necessary to use new resistance genes to breed resistant wheat varieties. The cultivation and growth of resistant wheat varieties are the most economical, effective, and environmental friendly methods to control stripe rust.
tritici ( Pst), is one of the most widespread and destructive fungal diseases of wheat worldwide. Stripe rust, caused by Puccinia striiformis f.
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